human il 2 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc human il 2
Human Il 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il+2/Human+IL-2+Recombinant+Protein/pm42881259-201-24-26
Average 93 stars, based on 20 article reviews

human il 2 - by Bioz Stars, 2026-10

93/100 stars

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Blocking Assay:

Article Title: Therapeutic potency and possible mechanism of Wuhu decoction underlying asthmatic progression via Th1/Th2 imbalance.
Article Snippet: Protein samples were boiled and separated by 10% sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE), and transferring to polyvinylidene difluoride (PVDF) membranes (Millipore, USA). .. After blocking with nonfat milk for 1 h, membranes were incubated overnight at 4 °C with the indicated primary antibodies from Cell Signaling Technology (Danvers, USA) at a 1:1,000 dilution, including IL-4 (#12227), IL-10 (#12163), GATA3 (#5852), IFN-γ (#8455), IL-2 (#12239), T-bet (#13232) and β-Actin (#3700). .. After washed, the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibody at a 1:5,000 dilution (#7074 or #7076, Cell Signaling Technology) for 2 h. The signals were developed with an electrochemical luminescence detection kit (Cat. 32106, Pierce Biotechnology, Waltham, USA) and analyzed by ImageJ software.

Incubation:

Article Title: Epidermodysplasia Verruciformis and Vδ2 γδ T-cell Expansion in STK4 Deficiency
Article Snippet: PBMCs were stained with 1 μM C-FSE (C34554, Thermo Fisher) at 37 °C for 20 min, washed three times, and then used to seed a U-bottom 96-well plate at a density of 1 × 10 6 cells/ml. .. The concentration of IL-2 (31058, Cell Signaling) in the medium was maintained at 50 ng/ml and cells were incubated with or without mitogens, including ImmunoCultTM Human CD3/CD28/CD2 T cell Activator (10970, STEMCELL) and phytohemagglutinin-M (PHA-M, Roche). .. After incubation for five days, the cell were incubated with anti-CD4-PE (300508, Biolegend) and anti-Vd2 TCR-BV750 (B6 clone, 747204, BD) antibodies, together with Aqua Live/Dead stain (L34966, Thermo Fisher), and analyzed on an LSRII (BD Biosciences).

Article Title: IL-2R-βγ binding compounds
Article Snippet: .. Three-fold serial dilutions of IL-2Rβγc ligands or IL-2 in starvation media were added to the cells and incubated for 30 min at 37° C. Cell extracts were prepared by adding a mixture of 10× Cell Lysis Buffer (Cell Signaling Technology #9803) and 1×HALT Phosphatase and Protease Inhibitor Cocktail (Thermo Fisher #78442) directly to the wells. .. TF-1β cells were derived from the growth factor-dependent human erythroleukemia cell line TF-1 (ATCC #CRL-2003), which naturally expresses IL-2Rγc but not IL-2Rβ.

Article Title: Dual IL-2R and IL-7R binding compounds
Article Snippet: TF-1 cells were derived from the growth factor-dependent human erythroleukemia cell line TF-1 (ATCC No. CRL-2003), which naturally expresses Rγc but not IL-2Rβ. .. Three-fold serial dilutions of IL-2Rβγc ligands or IL-2 in starvation media were added to the cells and incubated for 30 min at 37° C. Cell extracts were prepared by adding a mixture of 10× Cell Lysis Buffer (Cell Signaling Technology No. 9803) and 1×HALT Phosphatase and Protease Inhibitor Cocktail (Thermo Fisher No. 78442) directly to the wells. .. Three-fold serial dilutions of IL-2Rβγc ligands or IL-2 in starvation media were added to the cells and incubated for 30 min at 37° C. Cell extracts were prepared by adding a mixture of 10× Cell Lysis Buffer (Cell Signaling Technology No. 9803) and 1×HALT Phosphatase and Protease Inhibitor Cocktail (Thermo Fisher No. 78442) directly to the wells.

Concentration Assay:

Lysis:

Protease Inhibitor:

Cell Culture:

Article Title: Interferon Regulatory Factor 2 (IRF2) Suppression Enhances Murine Cardiac Allograft Survival Through Immune Evasion.
Article Snippet: The isolated cells were then analysed with flow cytometer (BD, NJ, USA). .. For the subsequent culture: the CD3+T cells obtained were cultured in RPMI 1640 medium (Hyclone), supplemented with 10% foetal bovine serum (04- 007- 1A, BI) and 100 IU/mL IL- 2 (68966, CST, MA, USA) and the culture medium was refreshed every 2–3 day. .. After stabilisation, T cells were co- cultured with HL1 cells at a ratio of 10:1 (HL1:T) and on the first day, 3 μg/mL anti- CD28 (BE0015- 1, BioXcell, NH, USA) was added to stimulate T cells.

Article Title: Interferon Regulatory Factor 2 ( IRF2 ) Suppression Enhances Murine Cardiac Allograft Survival Through Immune Evasion
Article Snippet: The isolated cells were then analysed with flow cytometer (BD, NJ, USA). .. For the subsequent culture: the CD3 + T cells obtained were cultured in RPMI 1640 medium (Hyclone), supplemented with 10% foetal bovine serum (04‐007‐1A, BI) and 100 IU/mL IL‐2 (68966, CST, MA, USA) and the culture medium was refreshed every 2–3 day. .. After stabilisation, T cells were co‐cultured with HL1 cells at a ratio of 10:1 (HL1:T) and on the first day, 3 μg/mL anti‐CD28 (BE0015‐1, BioXcell, NH, USA) was added to stimulate T cells.

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